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Antifungal activity of Cymbopogon winterianus jowitt ex bor against Candida albicans BJM
Oliveira,Wylly Araújo de; Pereira,Fillipe de Oliveira; Luna,Giliara Carol Diniz Gomes de; Lima,Igara Oliveira; Wanderley,Paulo Alves; Lima,Rita Baltazar de; Lima,Edeltrudes de Oliveira.
Candida albicans is an opportunistic yeast and a member of the normal human flora that commonly causes infections in patients with any type of deficiency of the immune system. The essential oils have been tested for antimycotic activity and pose much potential as antifungal agents. This work investigated the activity of the essential oil of Cymbopogon winterianus against C. albicans by MIC, MFC and time-kill methods. The essential oil (EO) was obtained by hydrodistillation using a Clevenger-type apparatus. It was tested fifteen strains of C. albicans. The MIC was determined by the microdilution method and the MFC was determined when an aliquot of the broth microdilution was cultivated in SDA medium. The phytochemical analysis of EO showed presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Antifungal; Cymbopogon winterianus.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200004
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Antimicrobial effectiveness of maleimides on fungal strains isolated from onychomycosis BABT
Gayoso,Carla Wanderley; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Cechinel Filho,Valdir; Trajano,Vinícius Nogueira; Pereira,Fillipe de Oliveira; Lima,Igara Oliveira.
This study aimed to analyze the effectiveness of maleimides as inhibitors on the growth of fungal strains isolated from onychomycosis by the solid medium diffusion procedure. The results showed a promising antifungical activity of the assayed maleimides with formation of fungal growth inhibition halos oscillating between diameter 10 and 23mm. MIC was 100µg/mL for 3,4-dichloro-N-phenyl-methyl-maleimide and 3,4-dichloro-N-phenyl-propil-maleimide and 200µg/mL for 3,4-dichloro-N-phenyl-maleimide, 3,4-dichloro-N-phenyl-ethyl-maleimide and 3,4-dichloro-N-phenyl-buthyl-maleimide.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Onychomycosis; Fungi; Maleimides; Antifungical activity.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500016
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Antimicrobial effectiveness of spices: an approach for use in food conservation systems BABT
Souza,Evandro Leite de; Stamford,Tânia Lúcia Montenegro; Lima,Edeltrudes de Oliveira; Trajano,Vinícius Nogueira; Barbosa Filho,José Maria.
There has been constant an increasing the search alternative and efficient compounds for food conservation, aiming a partial or total replacement of antimicrobial chemical additives. Spices offer a promising alternative for food safety. Inhibitory activity of spices and derivatives on the growth of bacteria, yeasts, fungi and microbial toxins synthesis has been well reported, so they could be used in food conservation as main or as adjuvant antimicrobial compounds in order to assure the production of microbiologically stable foods.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spices; Essential oil; Extracts; Chemical compounds; Antibacterial activity; Antifungal activity.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500007
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Chemical composition and antifungal activity of Hyptis suaveolens (L.) poit leaves essential oil against Aspergillus species BJM
Moreira,Ana Carolina Pessoa; Lima,Edeltrudes de Oliveira; Wanderley,Paulo Alves; Carmo,Egberto Santos; Souza,Evandro Leite de.
This study aimed to identify the constituents of the essential oil from Hyptis suaveolens (L.) leaves using a Gas Chromatograph -Mass Spectrometer and assess its inhibitory effect on some potentially pathogenic Aspergilli (A. flavus, A. parasiticus, A. ochraceus, A. fumigatus and A. niger). Eucaliptol (47.64 %) was the most abundant component in the oil, followed for gama-ellemene (8.15 %), beta-pynene (6.55 %), (+)3-carene (5.16 %), trans-beta-cariophyllene (4.69 %) and germacrene (4.86 %). The essential oil revealed an interesting anti-Aspergillus property characterized by a Minimum Inhibitory Concentration and Minimum Fungicidal Concentration of 40 and 80 µL/mL, respectively. The oil at 80 and 40 µL/mL strongly inhibited the mycelial growth of A....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hyptis suaveolens L. (Poit); Essential oil; Anti-Aspergillus effect.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100006
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Effect of cinnamomum zeylanicum blume essential oil on the rowth and morphogenesis of some potentially pathogenic Aspergillus species BJM
Carmo,Egberto Santos; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Sousa,Frederico Barbosa de.
Cinnamomum zeylanicum Blume is known for a wide range of medicinal properties. This study aimed to assess the interference of C. zeylanicum essential oil on the growth and morphogenesis of some potentially pathogenic Aspergillus species. The essential oil presented strong antifungal effect causing the growth inhibition of the assayed strains and development of large growth inhibition zones. MIC50 and MIC90 values were 40 and 80 µL/mL, respectively. 80, 40 and 20 µL/mL of the oil strongly inhibited the radial mycelial growth of A. niger, A. flavus and A. fumigatus along 14 days. 80 and 40 µL/mL of the oil caused a 100% inhibition of the fungal spore germination. Main morphological changes observed under light microscopy provided by the essential oil in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Cinnamomum zeylanicum Blume; Essential oil; Antifungal activity.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100021
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Effectiveness of Origanum vulgare L. and Origanum majorana L. essential oils in inhibiting the growth of bacterial strains isolated from the patients with conjunctivitis BABT
Oliveira,Jana Luíza Toscano Mendes de; Diniz,Margareth de Fátima Melo; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Trajano,Vinícius Nogueira; Santos,Bernadete Helena Cavalcante.
This study aimed to evaluate the antibacterial activity of Origanum vulgare L. and O. majorana L. essential oils on Staphylococcus aureus, S. coagulase negative, Enterobacter spp., Proteus spp., Acinetobacter spp., Klebsiella spp. isolated from the patients with conjunctivitis. The results showed a prominent inhibitory effect of both the essential oils on all the bacterial strains, noted by the large bacterial growth inhibition zones (15-32mm). The Minimum Inhibitory Concentrations (MIC) values were between 5-20µL/mL and 2.5-10 µL/mL for O. vulgare and O. majorana essential oil, respectively. The MIC were able to cause significant (P<0.05) inhibitory effect on the cell viability of Klebsiella spp., Proteus spp. and S. aureus providing a total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Origanum vulgare L.; Origanum marjorana L.; Essential oil; Antibacterial activity; Conjunctivitis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100006
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Effects of Cymbopogon winterianus Jowitt ex Bor essential oil on the growth and morphogenesis of Trichophyton mentagrophytes BJPS
Pereira,Fillipe de Oliveira; Wanderley,Paulo Alves; Viana,Fernando Antônio Cavalcanti; Lima,Rita Baltazar de; Sousa,Frederico Barbosa de; Santos,Sócrates Golzio dos; Lima,Edeltrudes de Oliveira.
Trichophyton mentagrophytes is a fungus causative agent of dermatophytosis, affecting humans worldwide. This has driven the search for products for the treatment of these infections. Accordingly, the aim of this study was to investigate the antifungal activity of the Cymbopogon winterianus essential oil against T. mentagrophytes. The antifungal tests consisted of antifungal screening, determination of MIC and MFC, analysis of the essential oil's effects on mycelial growth, germination of fungal spores, fungal viability, morphogenesis, cell wall (test with sorbitol) and cell membrane (cell leakage test) of T. mentagrophytes. Upon screening, the oil inhibited all strains, with zones of growth inhibition of 24-28 mm in diameter. The MIC was 312 μg/mL and CFM...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton mentagrophytes/mycology; Dermatophytosis Cymbopogon winterianus/essential oil; Dermatophytosis Cymbopogon winterianus/antifungal activity; Dermatophytes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100018
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Efficacy of Origanum essential oils for inhibition of potentially pathogenic fungi BJPS
Souza,Nadábia Almeida B; Lima,Edeltrudes de Oliveira; Guedes,Diego Nunes; Pereira,Fillipe de Oliveira; Souza,Evandro Leite de; Sousa,Frederico Barbosa de.
This study aimed to assess the efficacy of O. vulgare L. and O. majorana L. essential oil in inhibiting the growth and survival of potentially pathogenic fungal strains and also sought to evaluate the possible mechanisms involved in the establishment of the antifungal property of the tested essential oils through assays of osmotic stability and morphogenesis. Test strains included in this study were Candida albicans ATCC 7645, C. tropicalis LM-14, C. krusei LM-09, Cryptococcus neoformans FGF-5, Aspergillus flavus LM-02, A. fumigatus IPP-21, T. rubrum ATCC 28184, T. mentagrophytes LM-64, Microsporum gypseum ATCC 184, M. canis LM-36 and Cladosporium herbarium ATCC 26362. O. vulgare essential oil presented a MIC value of 80 µL/mL, while for O. majorana this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Pathogen fungi; Origanum sp/antifungal property.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300013
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Growth inhibition and morphological alterations of Trichophyton rubrum induced by essential oil from Cymbopogon winterianus Jowitt ex Bor BJM
Pereira,Fillipe de Oliveira; Wanderley,Paulo Alves; Viana,Fernando Antônio Cavalcanti; Lima,Rita Baltazar de; Sousa,Frederico Barbosa de; Lima,Edeltrudes de Oliveira.
Trichophyton rubrum is one of the most common fungi causer of dermatophytosis, mycosis that affect humans and animals around the world. Researches aiming new products with antifungal activity become necessary to overcome difficulties on treatment of these infections. Accordingly, this study aimed to investigate the antifungal activity of essential oil from Cymbopogon winterianus against the dermatophyte T. rubrum. The antifungal screening was performed by solid medium diffusion method with 16 T. rubrum strains, minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined using the microdilution method. The effects on mycelial dry weight and morphology were also observed. Screening showed essential oil in natura inhibited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton; Essential oil; Cymbopogon.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100029
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Inhibitory action of some essential oils and phytochemicals on the growth of various moulds isolated from foods BABT
Souza,Evandro Leite de; Lima,Edeltrudes de Oliveira; Freire,Kristerson Reinaldo de Luna; Sousa,Cristina Paiva de.
The aim of this study was to determine the sensitivity profile of mould strains isolated from foods to some essential oils and phytochemicals. The assayed mould strains were: Fusarium spp., Rhizopus spp., Aspergillus flavus, A. niger and Penicillium spp. According to results, Lippia alba N.E. Brown, Peumus boldus Molina, Lippia microphylla Phil., Citrus limon Risso and Cymbopogon citratus Stapf. essential oil and the phytochemicals citral, eugenol and mircene showed prominent antimould activity. Among the products that evidenced antimould activity, citral and eugenol showed the lowest minimum inhibitory concentrations, which was 1% and 4%, respectively, for the most of the tested mould strains.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant products; Antimould activity; Sensitivity profile.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200011
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Inhibitory effect of Cinnamomum zeylanicum Blume (Lauraceae) essential oil and beta-pinene on the growth of dematiaceous moulds BJM
Moreira,Ana Carolina Pessoa; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Van Dingenen,Monique Araújo; Trajano,Vinícius Nogueira.
Dematiaceous moulds are pathogen microorganisms able to act as etiological agents of mycoses with different degrees of severity. This study evaluated the effectiveness of Cinnamomum zeylanicum Blume essential oil and beta-pinene in inhibiting the growth of various strains of dematiaceous moulds (Alternaria brassicola, Cladosporium herbarium, C. resinae, C. cladosporioides, Chaetomum globosum, Curvularia sp., Fonsecaea compacta, Piedraia hortae). Antimicrobial assays were led by determining the Minimum Inhibitory Concentration-MIC using the solid medium diffusion procedure and observing the interference of the MIC values on the mould radial mycelial growth along 14 days. MIC values found to C. zeylanicum essential oil oscillated between 63 and 125 µL/mL....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dematiaceous moulds; Cinnamomum zeylanicum Blume; Beta-pinene; Antimould activity.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100008
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Isoflavone formononetin from red propolis acts as a fungicide against Candida sp BJM
Neves,Michelline Viviane Marques das; Silva,Tânia Maria Sarmento da; Lima,Edeltrudes de Oliveira; Cunha,Emídio Vasconcelos Leitão da; Oliveira,Eduardo de Jesus.
Abstract A bioassay-guided fractionation of two samples of Brazilian red propolis (from Igarassu, PE, Brazil, hereinafter propolis 1 and 2) was conducted in order to determine the components responsible for its antimicrobial activity, especially against Candida spp. Samples of both the crude powdered resin and the crude ethanolic extract of propolis from both locations inhibited the growth of all 12 tested Candida strains, with a minimum inhibitory concentration of 256 µg/mL. The hexane, acetate and methanol fractions of propolis 1 also inhibited all strains with minimum inhibitory concentration values ranging from 128 to 512 µg/mL for the six bacteria tested and from 32 to 1024 µg/mL for the yeasts. Similarly, hexane and acetate fractions of propolis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioassay-guided fractionation; Red propolis; Isoflavone; Formononetin; Candida.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100159
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Phytoconstituents from Sidastrum micranthum (A. St.-Hil.) Fryxell (Malvaceae) and antimicrobial activity of pheophytin a BJPS
Gomes,Roosevelt Albuquerque; Teles,Yanna Carolina Ferreira; Pereira,Fillipe de Oliveira; Rodrigues,Luis Alberto de Sousa; Lima,Edeltrudes de Oliveira; Agra,Maria de Fátima; Souza,Maria de Fátima Vanderlei de.
abstract Sidastrum micranthum (A. St.-Hil.) Fryxell, a member of the Malvaceae family, is called malva preta in Brazil. As this species is commonly used to treat bronchitis, cough, and asthma, better knowledge of its chemical compounds is important. The phytochemical study of its hexane extract, using chromatographic techniques, led to isolation of six compounds: the triterpene isoarborinol, a mixture of sitosterol and stigmasterol, sitosterol-3-O-β-D-glucopyranoside, pheophytin a, and 132-hydroxy-(132-S)-pheophytin a. Structural identification of these compounds was carried out using spectroscopic methods such as IR and 1D and 2D NMR (HOMOCOSY, HMQC, HMBC, and NOESY). Compounds isolated from S. micranthum were screened for their in vitro antifungal and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malvaceae/phytochemistry; Sidastrum micranthum/phytochemistry; Sidastrum micranthum/antimicrobial activity/in vitro study; Pheophytins; Medicinal plants..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400861
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Sensitivity of spoiling and pathogen food-related bacteria to Origanum vulgare L. (Lamiaceae) essential oil BJM
Souza,Evandro Leite de; Stamford,Tânia Lúcia Montenegro; Lima,Edeltrudes de Oliveira.
Origanum vulgare L. (oregano), Lamiaceae, has been known as plant specie with prominent biological properties for a long time. This study aimed to evaluate the antibacterial activity of Origanum vulgare essential oil on various Gram-positive and Gram-negative spoiling and/or pathogen food-related bacteria, as well as to observe its antimicrobial effectiveness in a food conservation micromodel. The results showed a strong antibacterial activity of the assayed essential oil noted by large growth inhibition zones (30-37 mm). MIC values were between 20-40 µL/mL for the most bacteria strains. Essential oil was able to cause significant (P<0.05) inhibitory effect on the bacteria viability providing a bacteriostatic effect after 24hours of exposure. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Origanum vulgare L.; Essential oil; Antimicrobial activity; Bacteria.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400023
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The potential of Origanum vulgare L. (Lamiaceae) essential oil in inhibiting the growth of some food-related Aspergillus species BJM
Carmo,Egberto Santos; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de.
Origanum vulgare L. (Lamiaceae) has been currently known for their interesting antimicrobial activity being regarded as alternative antimicrobial for use is food conservation systems. This study aimed to evaluate the effectiveness of O. vulgare essential oil in inhibiting the growth of some food-related Aspergillus species (A. flavus, A. parasiticus, A. terreus, A. ochraceus, A. fumigatus and A. niger). The essential oil revealed a strong anti-Aspergillus property providing an inhibition of all assayed mould strains. MIC values were between 80 and 20 µL/mL being found a MIC50 of 40 µL/mL. The essential oil at concentration of 80 and 40 µL/mL provided a fungicidal effect on A. flavus, A. fumigatus and A. niger noted by a total inhibition of the radial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: O. vulgare; Essential oil; Aspergillus.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200030
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